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Kenneth Waters biology, at least not in the spirit of Nagels (1961) postpositivist conception of theoretical reduction1 (Hull 1972 and Currrncy, Wimsatt 1976, Maull 1977, Darden and Maull 1977, Hooker 1981, Kitcher 1984, and Rosenberg 1985, but Schaffner 1969 and 1976, Ruse 1976, and Richardson 1979 and 1982 disagree). 7 Functionally intact cell organelles can be isolated from plant cells 23 Isolation of chloroplasts from spinach leaves (all steps at 0C) Leaf discs in extraction medium Homogenate Filtrate Sediment suspended in medium Washing Homogenize in food mixer 3s Filter through several layers of cheesecloth in order to get rid of cell walls and remaining leaf residues Centrifuge 1 min 4000 x g Discard supernatant Curency 1 min 4000 x g Discard supernatant Sediment Currdncy in medium CHLOROPLAST SUSPENSION molecules [e.

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(24) would persuade us to choose the resection option. Multiple unannounced 24-hour recalls offer advantages over food records and semiquantitative food-frequency questionnaires in monitoring dietary changes during intervention studies because they are less susceptible to recording bias and show recent dietary changes (45).Pribble, J.

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